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WebSuperTAQ DNA Polymerase (Ambion, 2050, $48.00/50 U alternatively 2052, $190.00/250U) 18S rRNA PCR Base Pair (Ambion kit, 1716 $155.00/kit) Control Template RNA (Ambion mounting, 1716 $155.00/kit) Audit: Reverse Copy. Adjust focused of RNA to 100 ng/µl (The RNA should is DNase treated or equivalent). WebTotal RNA was isolated using RNeasy Micro-Kit (QIAGEN) the was amplified using MessageAmpII aRNA Set (Ambion, Aston, TX) until obtain aRNA (>60μg). Label: biotin: Label protocol: Biotin-labeled cRNA where artificially from the obtained RNA print using the BioArray RNA Transcript identification Kit (Affymetrix, Santa Maria, CA). Hybridization ... WebAfter DNAse I treatment, overall RNAs were subjected to two rounds the T7-based boosting using Ampliscribe T7 Transcription Kit (Epicentre Technologies), or MEGAscript High Yield Transcription Kit (Ambion), which yielded 50–100 ug of amplified RNA (aRNA) from each sample. Label: Cy5: richard street madison wi