Phoss1
WebListeria monocytogenes is an intracellular pathogen transmitted to humans and animals through the consumption of contaminated food. It causes listeriosis and affects mostly at … WebConstruction of pHoss1 suicide plasmid [ 9 ]. Tetracycline-inducible antisense secY region ( anti - secY) was amplified from pIMAY and ligated with pMAD plasmid backbone. Abbreviations: MCS Multiple cloning site, ermC Erythromycin resistance gene, bla Beta-lactamase gene), ori Replication origin of the plasmid from pBR322
Phoss1
Did you know?
WebJul 30, 2024 · These fragments were then cloned into the digested pHoss1 vector and transformed into chemically competent E. coli DH5α. After sequencing, the resulting plasmids were electroporated into L. monocytogenes strain F2365 and selected in BHI plus erythromycin at 30 °C for 3 days. A single colony was streaked onto BHI without … WebpHoss1质粒价格1200元，pHoss1(爱迪基因#63158 )质粒图谱（map），质粒序列(Sequence)见下文，质粒抗性为氨苄青霉素，质粒大小为8995 ...
WebMay 31, 2015 · To assess the effectiveness of pHoss1 for the generation of stable in-frame deletion mutations, we deleted the ispG and ispH genes of L. monocytogenes serotype 4b strain F2365. Results showed that identification of the second allelic exchange mutants was very efficient with 80-100% of the colonies yielding desired deletion mutants. WebPurpose Listeria monocytogenes is a Gram-positive intracellular pathogen that cause listeriosis, a fatal foodborne disease for human and animals. Pathogenesis of L. monocytogenes is tightly...
WebThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies. pHoss1 (Plasmid #63158) Print Enlarge View all sequences Purpose Suicide plasmid for efficient gene mutation in Gram-positive bacteria Depositing Lab Attila Karsi Publication Abdelhamed et al Plasmid. 2015 May 31;81:1-8. doi: 10.1016/j.plasmid.2015.05.003. ( How to cite ) Sequence Information Sequences (7) Ordering
WebApr 11, 2024 · The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the …
WebJun 14, 2024 · To facilitate markerless gene deletion in Listeria monocytogenes, we developed a new suicide plasmid (pHoss1). pHoss1 contains the pMAD backbone, the … dgp railwayWebSep 1, 2015 · A novel plasmid (pHoss1) for Listeria monocytogenes was constructed. • pHoss1 is very efficient for the generation of in-frame deletion mutants. • The L. … dgp punjab address and email idWebFirst, the L. monocytogenes mutants with Man‐PTS IIC or IID deletion were constructed with the vector pHoss1. Then, the utilization of glucose and mannose and the sensitivity to durancin GL of the mutant strains were investigated. Afterward, the interactions between durancin GL and the subunits of IIC or IID in Man‐PTS of L. monocytogenes ... dgprcsWebAmenities. Blair Lofts Apartments PHASE 1 has 10 units. Blair Lofts Apartments PHASE 1 is currently renting between $1000 and $1199 per month, and offering 12 month lease terms. Blair Lofts Apartments PHASE 1 is located in Cincinnati, the 45229 zipcode, and the Cincinnati Public Schools. The full address of this building is 3401 Reading Rd ... dgp recordsWebApr 6, 2024 · Toronto Blue Jays. The first phase of the renovations at the 34-year-old Rogers Centre in Toronto opens five new neighborhoods and additional social spaces to any fan with a Blue Jays game ticket ... cicek teamWebMar 21, 2024 · In 2015, Congress instructed CMS to develop a master plan to address increased workload demands stemming from the Affordable Care Act. GSA and CMS … dg priority\u0027sWeb1 day ago · A. Lange & Söhne Lange 1 Moon Phase ref. 192.029. A timepiece that originates from a classic conception but flirts with contemporary design. One that has mastered the … ciceley carlisle